Genomics and Central Research Laboratory, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, India-563101.
Genomics and Central Research Laboratory, Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Tamaka, Kolar, India-563101; Centre for Nanoscience and Engineering, Indian Institute of Science, Bangalore, India-560012.
Diagn Microbiol Infect Dis. 2020 Jan;96(1):114923. doi: 10.1016/j.diagmicrobio.2019.114923. Epub 2019 Oct 24.
Leptospirosis is a recurring global disease of severe illness involving pulmonary and renal involvement in cattle and humans that needs attention to cure. The major challenge in treating leptospirosis disease is the diagnosis of the disease. The culturing of an organism is a gold standard for confirmation of the disease. The growth and optimistic identification of an organism require at least 8 to 14 days because of its slow growth characteristics. We have investigated various media conditions that are prepared based on the wealth of information obtained from 'omic' studies and report a sustainable Leptospira growth medium comprising of serum equivalent elements and nutrients for pyrimidine biosynthesis, which allows a visible growth of organism within 2 days.
钩端螺旋体病是一种反复发作的全球性疾病，可导致牛和人类肺部和肾脏受累，需要引起重视进行治疗。治疗钩端螺旋体病的主要挑战是该病的诊断。培养生物体是确认该疾病的金标准。由于其生长缓慢的特点，培养生物体的生长和乐观鉴定至少需要 8 到 14 天。我们研究了各种基于从“组学”研究中获得的丰富信息制备的培养基条件，并报告了一种可持续的钩端螺旋体生长培养基，其中包含血清等效物和嘧啶生物合成所需的营养物，这可以使生物体在 2 天内可见生长。
