从瑞士湖泊中分离出的嗜糖气单胞菌中质粒介导的喹诺酮耐药性
Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake.
作者信息
Picão Renata Cristina, Poirel Laurent, Demarta Antonella, Silva Carla Sofia Ferreira, Corvaglia Anna Rita, Petrini Orlando, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France.
出版信息
J Antimicrob Chemother. 2008 Nov;62(5):948-50. doi: 10.1093/jac/dkn341. Epub 2008 Sep 3.
OBJECTIVES
To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.
METHODS
Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.
RESULTS
A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes.
CONCLUSIONS
These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
目的
在从瑞士分离出的水生环境气单胞菌属中寻找质粒介导的qnr基因。
方法
对萘啶酸或环丙沙星最低抑菌浓度（MIC）≥1 mg/L的分离株，采用多重PCR方法并测序筛选qnr基因。通过转化转移质粒，并用PCR和测序对qnrS2基因周围的遗传结构进行进一步分析。
结果
从一株嗜麦芽糖气单胞菌分离株（卢加诺湖，卢加诺）中鉴定出一个qnrS2基因，该基因是位于一个广泛宿主范围的IncU型质粒上的一个可移动插入盒的一部分。该质粒还携带一个1类整合子，其中包含aac(6')-Ib-cr、bla(OXA-1)、catB3和arr-3基因盒。
结论
这些发现进一步强化了气单胞菌属作为环境中抗菌药物耐药决定因素储存库的作用。
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