Brentner Laura B, Mukherji Sachiyo T, Merchie Kate M, Yoon Jong Moon, Schnoor Jerald L, Van Aken Benoit
Department of Civil and Environmental Engineering, The University of Iowa, Iowa City, IA 52242.
Chemosphere. 2008 Oct;73(5):657-62. doi: 10.1016/j.chemosphere.2008.07.059.
Twelve Populus genes were identified from Arabidopsis thaliana sequences previously shown to be induced by exposure to 2,4,6-trinitrotoluene (TNT). Using the resources of the Poplar Genome Project and National Center for Biotechnology Information databases, Populus conserved domains were identified and used to design gene specific primers. RNA extracted from root tissues of TNT-exposed hydroponic poplar plants was used to quantify the expression of genes by reverse-transcriptase real-time polymerase chain reaction. Cyclophilin and 18S ribosomal DNA genes were used as internal standards. Exposure to TNT resulted in a significant increase of gene expression of two glutathione S-transferases (GST), peaking at levels of 25.0 +/- 13.1 and 10 +/- 0.7 fold the expression level of non-exposed plants after 24 h for each of the GST genes, respectively. This paper demonstrates the use of functional genomics information from the model plant species, Arabidopsis, to identify genes which may be important in detoxification of TNT in the model phytoremediation species, Populus trichocarpa.
从拟南芥序列中鉴定出12个杨树基因，这些序列先前已证明在暴露于2,4,6-三硝基甲苯（TNT）时会被诱导。利用杨树基因组计划和美国国立生物技术信息中心数据库的资源，鉴定出杨树保守结构域并用于设计基因特异性引物。从暴露于TNT的水培杨树植物根组织中提取的RNA用于通过逆转录实时聚合酶链反应定量基因表达。亲环蛋白和18S核糖体DNA基因用作内标。暴露于TNT导致两种谷胱甘肽S-转移酶（GST）的基因表达显著增加，每种GST基因在24小时后分别达到未暴露植物表达水平的25.0±13.1倍和10±0.7倍的峰值。本文展示了利用模式植物物种拟南芥的功能基因组学信息，来鉴定在模式植物修复物种毛果杨中对TNT解毒可能重要的基因。
