Mazari Aslam M A, Mannervik Bengt
Department of Neurochemistry, Stockholm University, SE-10691 Stockholm, Sweden.
Biochem Biophys Rep. 2015 Dec 4;5:141-145. doi: 10.1016/j.bbrep.2015.12.003. eCollection 2016 Mar.
The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) and the related 2,4-dinitrotoluene (DNT) are toxic environmental pollutants. The biotransformation and detoxication of these persistent compounds in higher organisms are of great significance from a health perspective as well as for the biotechnological challenge of bioremediation of contaminated soil. We demonstrate that different human glutathione transferases (GSTs) and GSTs from the fruit fly are catalysts of the biotransformation of TNT and DNT. The human GSTs had significant but modest catalytic activities with both DNT and TNT. However, GSTE6 and GSTE7 displayed outstanding high activities with both substrates.
硝基芳香族炸药2,4,6-三硝基甲苯（TNT）及相关的2,4-二硝基甲苯（DNT）是有毒的环境污染物。从健康角度以及受污染土壤生物修复的生物技术挑战来看，这些持久性化合物在高等生物中的生物转化和解毒具有重要意义。我们证明，不同的人类谷胱甘肽S-转移酶（GSTs）以及果蝇的GSTs是TNT和DNT生物转化的催化剂。人类GSTs对DNT和TNT均具有显著但适度的催化活性。然而，GSTE6和GSTE7对这两种底物均表现出极高的活性。
