用于通过电子顺磁共振脉冲电子双共振进行远程距离测量的氮氧化物自旋标记RNA
Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR.
作者信息
Frolow O, Endeward B, Schiemann O, Prisner T F, Engels J W
机构信息
Institut für Organische Chemie und Chemische Biologie (OCCB), Goethe-Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.
出版信息
Nucleic Acids Symp Ser (Oxf). 2008(52):153-4. doi: 10.1093/nass/nrn078.
PMID:18776299
Abstract
Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.
摘要
对RNA进行长距离测量有助于确定RNA折叠结构。在此，我们报告了通过“柱上合成”在尿嘧啶（U）、胞嘧啶（C）和腺嘌呤（A）位点特异性掺入氮氧化物自旋标记。成功地对几种长度在2至6纳米范围内的RNA进行了脉冲电子双共振（PELDOR）测量。
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