Hladká M, Altaner C
J Natl Cancer Inst. 1976 Aug;57(2):283-7. doi: 10.1093/jnci/57.2.283.
The avian sarcoma virus genome (Schmidt-Ruppin strain) in transformed hamster cells resistant to 8-azaquanine [Ha(SR)AG-50] was strongly suppressed. The suppression was genetically stable and could not be overcome by attempts at induction with 5-iodo-2'-deoxyuridine. Fusion of hamster cells, which had suppressed virus genome, with chicken Rous-associated virus (RAV-1)-preinfected cells easily rescued the sarcoma virus. The rescued virus had envelope properties of RAV-1, as determined by viral interference, virus neutralization, and plating on genetically resistant chicken cells. By repeatedly cloning the rescued virus, we determined that virus recombined in the rescue experiment and that the recombinant virus had the envelope properties of helper virus used for its rescue. Cells with suppressed avian sarcoma virus genome were suitable for preparation of different recombinant viruses.
在对8-氮杂鸟嘌呤具有抗性的转化仓鼠细胞[Ha(SR)AG-50]中，禽肉瘤病毒基因组（施密特-鲁平株）受到强烈抑制。这种抑制在遗传上是稳定的，用5-碘-2'-脱氧尿苷诱导也无法克服。将已抑制病毒基因组的仓鼠细胞与预先感染鸡劳氏相关病毒（RAV-1）的细胞融合，很容易拯救出肉瘤病毒。通过病毒干扰、病毒中和以及在遗传抗性鸡细胞上进行铺板测定，拯救出的病毒具有RAV-1的包膜特性。通过对拯救出的病毒进行反复克隆，我们确定在拯救实验中病毒发生了重组，并且重组病毒具有用于拯救它的辅助病毒的包膜特性。具有被抑制的禽肉瘤病毒基因组的细胞适合用于制备不同的重组病毒。
