Li Chun-Xiao, Jiang Mei-Shan, Chen Shi-Yi, Lai Song-Jia
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.
Yi Chuan. 2008 Jul;30(7):893-9. doi: 10.3724/sp.j.1005.2008.00893.
Single nucleotide polymorphism (SNP) in exon 1 and 3 of fibroblast growth factor (FGF5) gene was studied by DNA sequencing in Yingjing angora rabbit, Tianfu black rabbit and California rabbit. A frameshift mutation (TCT insert) at base position 217 (site A) of exon 1 and a T/C missense mutation at base position 59 (site B) of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 (site C) of exon 3 was found with similar frequency in three rabbit breeds. Least square analysis showed that different genotypes had no significant association with wool yield in site A, and had high significant association with wool yield in site B (P<0.01) and significant association with wool yield in site C (P<0.05). It was concluded from the results that FGF5 gene could be the potential major gene affecting wool yield or link with the major gene, and polymorphic loci B and C may be used as molecular markers for im-proving wool yield in angora rabbits.
采用DNA测序技术，对英系安哥拉兔、天府黑兔和加利福尼亚兔的成纤维细胞生长因子（FGF5）基因外显子1和外显子3的单核苷酸多态性（SNP）进行了研究。在英系安哥拉兔中，外显子1第217位碱基处（位点A）发现了一个移码突变（插入TCT），外显子3第59位碱基处（位点B）发现了一个T/C错义突变，且频率较高；在3个兔品种中外显子3第3位碱基处（位点C）发现了频率相近的T/C同义突变。最小二乘分析表明，位点A不同基因型与产毛量无显著关联，位点B不同基因型与产毛量极显著相关（P<0.01），位点C不同基因型与产毛量显著相关（P<0.05）。结果表明，FGF5基因可能是影响产毛量的潜在主基因或与主基因连锁，多态位点B和C可作为提高安哥拉兔产毛量的分子标记。
