Chen S J, Liu T, Liu Y J, Dong B, Huang Y T, Gu Z L
Mountain Area Research Institute, Agricultural University of Hebei, Baoding, Hebei, PR China.
Genet Mol Res. 2011 Nov 4;10(4):3365-70. doi: 10.4238/2011.November.4.1.
The Rex rabbit is a typical fur breed. Wool density, hair length, wool fineness, and hide area are the main indices of fur quality. We previously found that the CCNA2 gene plays an important role in hair follicle initiation and development, and it is involved in the distinctive wool density of the Rex rabbit. It is an important candidate gene for wool density selection through marker-assisted selection. We conducted an association study to identify single nucleotide polymorphisms (SNPs) within the CCNA2 gene and their ligands associated with wool density. Using PCR-RFLP technology, we discovered two SNPs (129G>A and 1140G>C) of the CCNA2 gene. Allele frequencies of these two SNPs were investigated and evaluated by the χ(2) test in 100 Rex rabbits. The two SNPs were both in Hardy-Weinberg equilibrium. We also looked for a potential association of these SNPs with fur traits in 100 Rex rabbits. Rex rabbits with the GG genotype had significantly higher wool density (P < 0.01) than those with other genotypes; the other three fur traits did not differ significantly among the genotypes. In conclusion, the two SNPs of the CCNA2 gene affect wool density in the Rex rabbit.
獭兔是典型的皮用品种。毛密度、毛长度、毛细度和皮面积是毛皮品质的主要指标。我们之前发现CCNA2基因在毛囊起始和发育中起重要作用，且与獭兔独特的毛密度有关。它是通过标记辅助选择进行毛密度选择的重要候选基因。我们进行了一项关联研究，以鉴定CCNA2基因内的单核苷酸多态性（SNP）及其与毛密度相关的配体。利用PCR-RFLP技术，我们发现了CCNA2基因的两个SNP（129G>A和1140G>C）。通过χ(2)检验对100只獭兔中这两个SNP的等位基因频率进行了调查和评估。这两个SNP均处于哈迪-温伯格平衡。我们还在100只獭兔中寻找了这些SNP与毛皮性状的潜在关联。GG基因型的獭兔毛密度显著高于其他基因型的獭兔（P < 0.01）；其他三个毛皮性状在各基因型之间没有显著差异。总之，CCNA2基因的两个SNP影响獭兔的毛密度。
