Anitua Eduardo, Orive Gorka, Pla Rafael, Roman Pedro, Serrano Victoriano, Andía Isabel
Biotechnology Institute I MAS D, San Antonio 15, 01005 Vitoria, Spain.
J Biomed Mater Res A. 2009 Oct;91(1):158-65. doi: 10.1002/jbm.a.32217.
The effect of local application of scaffold-like preparation rich in growth factors (PRGF) on bone regeneration in artificial defects and the potential effect of humidifying titanium dental implants with liquid PRGF on their osseointegration were investigated. The PRGF formulations were obtained from venous blood of three goats and applied either as a 3D fibrin scaffold (scaffold-like PRGF) in the regeneration of artificial defects or as liquid PRGF via humidifying the implants before their insertion. Initially, 12 defects were filled with scaffold-like PRGF and another 12 were used as controls. The histological analysis at 8 weeks revealed mature bone trabeculae when PRGF was used, whereas the control samples showed mainly connective tissue with incipient signs of bone formation. For the second set of experiments, 26 implants (13 humidified with liquid PRGF) were placed in the tibiae of goats. Histological and histomorphometric results demonstrated that application of liquid PRGF increased the percentage of bone-implant contact in 84.7%. The whole surface of the PRGF-treated implants was covered by newly formed bone, whereas only the upper half was surrounded in control implants. In summary, PRGF can accelerate bone regeneration in artificial defects and improve the osseointegration of titanium dental implants.
研究了局部应用富含生长因子的支架样制剂（PRGF）对人工骨缺损骨再生的影响，以及用液体PRGF湿润钛牙种植体对其骨整合的潜在影响。PRGF制剂取自三只山羊的静脉血，以3D纤维蛋白支架（支架样PRGF）的形式应用于人工骨缺损的再生，或在植入前通过湿润种植体以液体PRGF的形式应用。最初，12个骨缺损填充支架样PRGF，另外12个作为对照。8周时的组织学分析显示，使用PRGF时可见成熟的骨小梁，而对照样本主要显示为有早期骨形成迹象的结缔组织。在第二组实验中，将26个种植体（13个用液体PRGF湿润）植入山羊胫骨。组织学和组织形态计量学结果表明，应用液体PRGF使骨-种植体接触百分比增加了84.7%。PRGF处理的种植体整个表面被新形成的骨覆盖，而对照种植体仅上半部分被骨包围。总之，PRGF可加速人工骨缺损的骨再生并改善钛牙种植体的骨整合。
