Cannon T M, Koskela R J, Burks C, Stallings R L, Ford A A, Hempfner P E, Brown H T, Fickett J W
Los Alamos National Laboratory, NM 87545.
Biotechniques. 1991 Jun;10(6):764-7.
SCORE, a program for computer-assisted scoring of Southern blots of clone DNA, retains the use of expert human judgment while taking over much of the drudgery of the scoring task. The primary functions of the program are to help make an aligned overlay of the fluorescence gel image and the autoradiogram blot image, to keep track of band and lane locations and to store the resulting data directly into a database. Use of SCORE has resulted in greatly increased efficiency and accuracy.
SCORE是一个用于对克隆DNA的Southern印迹进行计算机辅助评分的程序,它在承担评分任务中大部分繁重工作的同时,仍保留了专家的人工判断。该程序的主要功能是帮助生成荧光凝胶图像和放射自显影片印迹图像的对齐叠加图,跟踪条带和泳道位置,并将结果数据直接存储到数据库中。使用SCORE大大提高了效率和准确性。