Mazid M A, Kaplan M
CHEMBIOMED LTD., Edmonton Research Park, Alberta, Canada.
Bioconjug Chem. 1991 Jan-Feb;2(1):32-7. doi: 10.1021/bc00007a006.
Immunoadsorbents with a synthetic oligosaccharide hapten representing human blood group A specific substances are prepared. The synthetic hapten, known as A-trisaccharide, which carries a space arm, is chemically attached to various solid supports, either directly through a suitable functional group at the end of the spacer arm or indirectly via a protein conjugated to the hapten. The preparation involves simple and mild procedures for the activation and/or derivatization of the supports. The latter includes naturally occurring polyhydroxy materials such as agarose, cellulose, or cellulose derivatives, and other particulate materials such as inorganic diatomites and a synthetic organic copolymer. The methods used for the coupling concern specifically the preparation of controlled-capacity and high-efficiency immunoadsorbents, with limited incorporations, which may be prepared easily and used for the selective removal, or affinity chromatographic separation, of specific antibodies from plasma environment or blood. It has been found that while hapten incorporation to the support may be varied rather easily, the physical nature of the support as well as the form of the hapten is important in determining the efficiency of an immunoadsorbent.
制备了带有代表人类 A 血型特异性物质的合成寡糖半抗原的免疫吸附剂。这种被称为 A-三糖的合成半抗原带有一个间隔臂，它通过间隔臂末端的合适官能团直接化学连接到各种固体载体上，或者通过与半抗原偶联的蛋白质间接连接。制备过程涉及用于载体活化和/或衍生化的简单温和程序。后者包括天然存在的多羟基材料，如琼脂糖、纤维素或纤维素衍生物，以及其他颗粒材料，如无机硅藻土和合成有机共聚物。用于偶联的方法特别涉及制备具有可控容量和高效率的免疫吸附剂，其掺入量有限，可以很容易地制备并用于从血浆环境或血液中选择性去除特定抗体或进行亲和色谱分离。已经发现，虽然半抗原掺入载体相对容易变化，但载体的物理性质以及半抗原的形式对于确定免疫吸附剂的效率很重要。
