Kolar C, Knödler U, Seiler F R
Research Laboratories of Behringwerke AG, Marburg/Lahn, W. Germany.
Behring Inst Mitt. 1988 Apr(82):94-103.
The carbohydrate structures determining the ABO blood group system have been almost completely characterized. Recent advances in the stereoselective synthesis of such complex structures have made it possible to prepare pure oligosaccharides in large quantities and to study their possible uses for diagnostics and pharmaceutical processing techniques. In the experiments described here A and B blood group specific determinants were synthesized and bound to solid phases by means of suitable spacers in order to study their use as immunoadsorbents. The objective was to adsorb blood group specific antibodies from positive sera and from immunoglobulin preparations. It was shown that the anti-A and anti-B antibodies bound to the immunoadsorbents with high affinity could be removed effectively. The effects were achieved both using affinity chromatography and "batch processing".
决定ABO血型系统的碳水化合物结构已几乎完全被表征。此类复杂结构立体选择性合成的最新进展使得大量制备纯寡糖并研究其在诊断和药物加工技术中的潜在用途成为可能。在此处描述的实验中，合成了A和B血型特异性决定簇，并通过合适的间隔物将其连接到固相上，以研究它们作为免疫吸附剂的用途。目的是从阳性血清和免疫球蛋白制剂中吸附血型特异性抗体。结果表明，与免疫吸附剂以高亲和力结合的抗A和抗B抗体能够被有效去除。使用亲和色谱法和“批量处理”均实现了上述效果。
