National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
J Clin Microbiol. 2012 Feb;50(2):460-4. doi: 10.1128/JCM.06090-11. Epub 2011 Nov 23.
We have developed a novel microsphere-based genotyping method for 46 mucosal human papillomavirus (HPV) types. HPV DNA was amplified by PCR using general primers and typed by hybridization to HPV type-specific probes coupled to sortable microspheres based on the Luminex xMAP technology. Hybridization to each probe was specific for each HPV type without cross-hybridization and sensitive enough to allow typing of HPV contained in clinical specimens. The method was validated with direct sequencing and the Roche Linear Array genotyping method.
我们开发了一种新的基于微球的 46 种黏膜型人乳头瘤病毒（HPV）分型方法。HPV DNA 通过使用通用引物的 PCR 扩增，并通过与 HPV 型特异性探针的杂交进行分型，这些探针连接到基于 Luminex xMAP 技术的可分选微球上。与每个探针的杂交是针对每个 HPV 型的，没有交叉杂交，并且足够灵敏，可以对临床标本中包含的 HPV 进行分型。该方法通过直接测序和罗氏线性阵列基因分型方法进行了验证。
