Xie Min, Hu Jun, Long Yan-Min, Zhang Zhi-Ling, Xie Hai-Yan, Pang Dai-Wen
College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.
Biosens Bioelectron. 2009 Jan 1;24(5):1311-7. doi: 10.1016/j.bios.2008.07.058. Epub 2008 Aug 5.
Nanomaterial-based nanobiosensors (nanobiodevices or nanobioprobes) are increasingly emphasized. Here, quantum dots and gamma-Fe(2)O(3) magnetic nanoparticles were co-embedded into single swelling poly(styrene/acrylamide) copolymer nanospheres to fabricate fluorescent-magnetic bifunctional nanospheres. Subsequently, fluorescent-magnetic-biotargeting trifunctional nanobiosensors (TFNS) modified with wheat germ agglutinin (WGA), peanut agglutinin (PNA) or Dolichos biflorus agglutinin (DBA) were conveniently produced so as to bind with A549 cells which are surface-expressed with N-acetylglucosamine, d-galactosamine and N-acetylgalactosamine residues. The values of WGA, PNA and DBA on each nanobiosensor were calculated to be 40, 14 and 60, respectively. These three kinds of lectin-modified trifunctional nanobiosensors (lectin-TFNS) can be used for qualitative and quantitative analysis of the glycoconjugates on A549 cell surface. The fluorescence intensity of WGA-modified nanobiosensors related to N-acetylglucosamine on A549 cell surface was much higher than that of PNA-modified nanobiosensors corresponding to d-galactosamine and that of N-acetylgalactosamine-related DBA-modified nanobiosensors, which is consistent with the results detected by flow cytometry. Lectin-modified trifunctional nanobiosensors not only can quantify the different glycoconjugates on A549 cell surface, but also can recognize and isolate A549 cells. 0.5mg of WGA-modified fluorescent-magnetic trifunctional nanobiosensors could capture 7.0 x 10(4) A549 cells. Therefore, the lectin-modified trifunctional nanobiosensors may be applied in mapping the glycoconjugates on cell surfaces and for recognition and isolation of targeted cells.
基于纳米材料的纳米生物传感器（纳米生物器件或纳米生物探针）正受到越来越多的关注。在此，将量子点和γ-Fe(2)O(3)磁性纳米颗粒共嵌入单个溶胀的聚（苯乙烯/丙烯酰胺）共聚物纳米球中，以制备荧光-磁性双功能纳米球。随后，方便地制备了用小麦胚芽凝集素（WGA）、花生凝集素（PNA）或双花扁豆凝集素（DBA）修饰的荧光-磁性-生物靶向三功能纳米生物传感器（TFNS），以便与表面表达N-乙酰葡糖胺、D-半乳糖胺和N-乙酰半乳糖胺残基的A549细胞结合。计算得出每个纳米生物传感器上WGA、PNA和DBA的值分别为40、14和60。这三种凝集素修饰的三功能纳米生物传感器（凝集素-TFNS）可用于对A549细胞表面糖缀合物进行定性和定量分析。WGA修饰的纳米生物传感器与A549细胞表面N-乙酰葡糖胺相关的荧光强度远高于PNA修饰的纳米生物传感器对应D-半乳糖胺的荧光强度以及与N-乙酰半乳糖胺相关的DBA修饰的纳米生物传感器的荧光强度，这与流式细胞术检测结果一致。凝集素修饰的三功能纳米生物传感器不仅可以定量A549细胞表面不同的糖缀合物，还可以识别和分离A549细胞。0.5mg的WGA修饰的荧光-磁性三功能纳米生物传感器可以捕获7.0×10(4)个A549细胞。因此，凝集素修饰的三功能纳米生物传感器可应用于绘制细胞表面的糖缀合物以及识别和分离靶向细胞。
