Oral and Maxillofacial Pathology Dept. School of Dentistry, Ardabil University of Medical Sciences (ArUMS), Ardabil, Iran.
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Arch Oral Biol. 2021 May;125:105088. doi: 10.1016/j.archoralbio.2021.105088. Epub 2021 Feb 19.
We aimed to assess the effect of quercetin as one of the most common polyphenols with anti-inflammatory and antioxidant properties on expression levels of catalase (CAT), superoxide dismutase 1 (SOD1), and glutathione peroxidase 1 (GPX1), involved in the detoxification of reactive oxygen species (ROS), and histology of dental pulp in streptozotocin-diabetic rats.
Type 1 diabetes mellitus (T1DM) was induced by intraperitoneal injection of streptozotocin in adult male Wistar rats. Animals (n = 24) were equally distributed into control, diabetes, and diabetes treated with quercetin groups. Rats were gavaged daily with quercetin (25 mg/kg) for forty days. To measure the mRNA levels of antioxidant genes, quantitate real-time PCR was applied. The oxidative stress parameters such as total antioxidant capacity (TAC) and histopathological assessments were performed.
A significant increase in the relative quantification mRNA levels of SOD1, CAT, GPX1 was detected in diabetic rat dental pulp. Besides, persistent hyperglycemia led to the enhancement of TAC level and degeneration of connective tissue of the dental pulp. Interestingly, quercetin normalized the expression mRNA levels of CAT, SOD1, GPX1 to near the normal level. Moreover, quercetin treatment normalized TAC levels.
Because of the crucial role of antioxidants in diabetic complications, the findings of the current study presented a molecular basis for the protective effect of quercetin on dental pulp in diabetic conditions.
我们旨在评估槲皮素作为具有抗炎和抗氧化特性的最常见多酚之一对过氧化氢酶 (CAT)、超氧化物歧化酶 1 (SOD1) 和谷胱甘肽过氧化物酶 1 (GPX1) 表达水平的影响，这些酶参与活性氧 (ROS) 的解毒，以及链脲佐菌素诱导的糖尿病大鼠牙髓组织学。
通过腹腔注射链脲佐菌素诱导成年雄性 Wistar 大鼠 1 型糖尿病 (T1DM)。动物 (n=24) 平均分为对照组、糖尿病组和糖尿病用槲皮素治疗组。大鼠每天用槲皮素 (25mg/kg) 灌胃 40 天。为了测量抗氧化基因的 mRNA 水平，应用定量实时 PCR。进行了氧化应激参数，如总抗氧化能力 (TAC) 和组织病理学评估。
糖尿病大鼠牙髓中 SOD1、CAT、GPX1 的相对定量 mRNA 水平显著增加。此外，持续的高血糖导致牙髓结缔组织的 TAC 水平升高和退化。有趣的是，槲皮素使 CAT、SOD1、GPX1 的 mRNA 表达水平正常化接近正常水平。此外，槲皮素治疗使 TAC 水平正常化。
由于抗氧化剂在糖尿病并发症中的关键作用，本研究的结果为槲皮素在糖尿病条件下对牙髓的保护作用提供了分子基础。
