Fölling Jonas, Bossi Mariano, Bock Hannes, Medda Rebecca, Wurm Christian A, Hein Birka, Jakobs Stefan, Eggeling Christian, Hell Stefan W
Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.
Nat Methods. 2008 Nov;5(11):943-5. doi: 10.1038/nmeth.1257. Epub 2008 Sep 15.
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.
我们介绍了一种基于普通荧光团的远场荧光纳米显微镜技术，该技术通过将大多数荧光团切换到亚稳态暗态（如三重态），并计算剩余荧光团或自发回到基态的荧光团的位置来实现。用单激光进行连续宽场照明以及使用连续工作的相机，获得了罗丹明和荧光蛋白标记的（活）样品的双色图像，证明了这是一种简单而强大的超分辨率方法。
