Cluster parameter-based DBSCAN maps for image characterization.

Single-molecule localization microscopy techniques are one of the most powerful methods in biological studies, allowing the visualization of nanoclusters. Cluster analysis algorithms are used for quantitative evaluation, with DBSCAN being one of the most widely used. Clustering results are extremely sensitive to the initial parameters; thus, several methods including DBSCAN maps, have been developed for parameter optimization. Here, we introduce cluster parameter-based DBSCAN maps, which are directly applicable to measured datasets. These maps can be used for image characterization and parameter optimization through sensitivity studies. We show the applicability of these maps to simulated and measured datasets and compare our results with the recently implemented lacunarity analysis for SMLM measurements.