Chmura A, Shapovalova A A, van Pelt S, van Rantwijk F, Tourova T P, Muyzer G, Sorokin D Yu
Biocatalysis & Organic Chemistry, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Appl Microbiol Biotechnol. 2008 Nov;81(2):371-8. doi: 10.1007/s00253-008-1685-x. Epub 2008 Sep 16.
An enrichment culture from saline soda soils, using acetate as carbon and energy source and 2-phenylpropionitrile as nitrogen source (PPN) at pH 10, resulted in the isolation of strain ANL-alpha CH3. The strain was identified as a representative of the genus Halomonas in the Gammaproteobacteria. The bacterium was capable of PPN utilization as a nitrogen source only, while phenylacetonitrile (PAN) served both as carbon, energy and nitrogen source. This capacity was not described previously for any other haloalkaliphilic bacteria. Apart from the nitriles mentioned above, resting cells of ANL-alpha CH3 also hydrolyzed mandelonitrile, benzonitrile, acrylonitrile, and phenylglycinonitrile, presumably using nitrilase pathway. Neither nitrile hydratase nor amidase activity was detected. The isolate showed a capacity to grow with benzoate and salicylate as carbon and energy source and demonstrated the ability to completely mineralize PAN. These clearly indicated a potential to catabolize aromatic compounds. On the basis of unique phenotype and distinct phylogeny, strain ANL-alpha CH3 is proposed as a novel species of the genus Halomonas--Halomonas nitrilicus sp. nov.
从盐碱土中富集培养，以乙酸盐作为碳源和能源，在pH值为10的条件下以2-苯基丙腈作为氮源（PPN），分离得到菌株ANL-α CH3。该菌株被鉴定为γ-变形菌纲嗜盐单胞菌属的代表。该细菌仅能利用PPN作为氮源，而苯乙腈（PAN）可作为碳源、能源和氮源。此前未在任何其他嗜盐碱细菌中描述过这种能力。除上述腈类化合物外，ANL-α CH3的静息细胞也能水解扁桃腈、苯甲腈、丙烯腈和苯甘氨腈，推测是通过腈水解酶途径。未检测到腈水合酶或酰胺酶活性。该分离株显示出以苯甲酸盐和水杨酸盐作为碳源和能源生长的能力，并证明能够完全矿化PAN。这些清楚地表明其具有分解代谢芳香族化合物的潜力。基于独特的表型和明显的系统发育关系，菌株ANL-α CH3被提议为嗜盐单胞菌属的一个新物种——嗜腈嗜盐单胞菌（Halomonas nitrilicus sp. nov.）。
