Pereira Aline C, Oliveira Denilson F, Silva Geraldo H, Figueiredo Henrique C P, Cavalheiro Albero J, Carvalho Douglas A, Souza Luciana P, Chalfoun Sára M
Departamento de Química, Universidade Federal de Lavras, Lavras, Minas Gerais, Brazil.
An Acad Bras Cienc. 2008 Sep;80(3):427-32. doi: 10.1590/s0001-37652008000300004.
To find out natural antimicrobial agents as alternative in therapeutics and to preserve food, the methanol extract of Solanum palinacanthum aerial parts was submitted to purification steps guided by antibacterial and antifungal assays. As a consequence, the flavonoid rutin and 3,5-dicaffeoylquinic acid were isolated by column chromatography and high performance liquid chromatography, and identified by mass and nuclear magnetic resonance spectrometry. Minimal inhibitory concentrations (MIC) of the quinic acid derivative against Aeromonas hydrophila, Bacillus subtilis, Staphylococcus aureus and the fungus Aspergillus ochraceus were 250, 1000, 1000 and > 568 microg/mL, respectively. Against the same microorganisms, MIC for rutin were 1000, > 1000, > 1000 and 35 microg/mL, respectively. Rutin was very promising for A. ochraceus control, since its MIC against such fungus was close to the one observed for benzalkonium chloride, which is used as a fungicide in Brazil.
为了寻找可替代的天然抗菌剂用于治疗和食品保鲜，对刺萼龙葵地上部分的甲醇提取物进行了抗菌和抗真菌试验指导下的纯化步骤。结果，通过柱色谱和高效液相色谱法分离出了黄酮类芦丁和3,5 - 二咖啡酰奎宁酸，并通过质谱和核磁共振光谱进行了鉴定。奎宁酸衍生物对嗜水气单胞菌、枯草芽孢杆菌、金黄色葡萄球菌和真菌赭曲霉的最低抑菌浓度（MIC）分别为250、1000、1000和>568微克/毫升。针对相同的微生物，芦丁的MIC分别为1000、>1000、>1000和35微克/毫升。芦丁对赭曲霉的控制非常有前景，因为其对该真菌的MIC接近巴西用作杀菌剂的苯扎氯铵的MIC。
