Pejić Snezana, Todorović Ana, Stojiljković Vesna, Cvetković Dragana, Lucić Nenad, Radojicić Ratko M, Saicić Zorica S, Pajović Snezana B
Laboratory of Molecular Biology and Endocrinology, Vinca Institute of Nuclear Sciences, Belgrade, Serbia.
An Acad Bras Cienc. 2008 Sep;80(3):515-22. doi: 10.1590/s0001-37652008000300011.
Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes in activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed with uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy subjects. Decrease of both SOD activity and level was found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders, render these patients more susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.
流行病学和实验数据表明，氧衍生自由基参与了妇科疾病的发病机制以及癌症的发展。本研究的目的是检测被诊断为子宫肌瘤、子宫内膜息肉、单纯性增生、复杂性增生和子宫内膜腺癌的患者血液和子宫内膜组织中铜/锌超氧化物歧化酶（CuZnSOD）的活性和水平以及脂质氢过氧化物（LOOH）的变化。我们的研究结果显示，与健康受试者相比，所有受试患者血液中的超氧化物歧化酶活性降低，而超氧化物歧化酶蛋白水平未变。与患有息肉或肌瘤的女性相比，单纯性增生、复杂性增生和腺癌患者的子宫内膜中超氧化物歧化酶活性和水平均降低。与健康受试者或诊断为良性疾病的患者相比，增生或腺癌患者的两种组织中脂质氢过氧化物水平均升高。我们的研究结果表明，妇科疾病患者中超氧化物歧化酶活性和水平的降低以及脂质氢过氧化物水平的升高，使这些患者更容易受到活性氧（ROS）引起的氧化损伤。活性氧生成与超氧化物歧化酶水平的失衡可能在妇科患者组织损伤的发病机制和/或持续过程中起重要作用。由于有证据表明超氧化物歧化酶可能是癌症治疗的一个靶点，我们的研究结果为进一步研究和临床应用选择提供了依据。
