Cormio Antonella, Guerra Flora, Cormio Gennaro, Pesce Vito, Fracasso Flavio, Loizzi Vera, Resta Leonardo, Putignano Giuseppe, Cantatore Palmiro, Selvaggi Luigi Eustacchio, Gadaleta Maria Nicola
Department of Biosciences, Biotechnologies and Pharmacological Sciences, University of Bari, Via Orabona, Bari, 4-70126, Italy.
BMC Res Notes. 2012 Jun 7;5:279. doi: 10.1186/1756-0500-5-279.
An increase in mitochondrial DNA (mtDNA) content and mitochondrial biogenesis associated with the activation of PGC-1α signalling pathway was previously reported in type I endometrial cancer. The aim of this study has been to evaluate if mtDNA content and the citrate synthase (CS) activity, an enzyme marker of mitochondrial mass, increase in progression from control endometrium to hyperplasia to type I endometrial carcinoma.
Given that no statistically significant change in mtDNA content and CS activity in endometrium taken from different phases of the menstrual cycle or in menopause was found, these samples were used as control. Our research shows, for the first time, that mtDNA content and citrate synthase activity increase in hyperplastic endometrium compared to control tissues, even if their levels remain lower compared to cancer tissue. In particular, mtDNA content increases seem to precede increases in CS activity. No statistically significant change in mtDNA content and in CS activity was found in relation to different histopathological conditions such as grade, myometrial invasion and stage.
MtDNA content and citrate synthase activity increases in pre-malignant lesions could be a potential molecular marker for progression from hyperplasia to carcinoma.
先前报道在I型子宫内膜癌中，线粒体DNA（mtDNA）含量增加以及与PGC-1α信号通路激活相关的线粒体生物合成增加。本研究的目的是评估从对照子宫内膜到增生再到I型子宫内膜癌进展过程中，mtDNA含量和线粒体质量的酶标志物柠檬酸合酶（CS）活性是否增加。
鉴于在取自月经周期不同阶段或绝经后的子宫内膜中未发现mtDNA含量和CS活性有统计学显著变化，这些样本用作对照。我们的研究首次表明，与对照组织相比，增生性子宫内膜中的mtDNA含量和柠檬酸合酶活性增加，尽管其水平仍低于癌组织。特别是，mtDNA含量的增加似乎先于CS活性的增加。在不同组织病理学状况如分级、肌层浸润和分期方面，未发现mtDNA含量和CS活性有统计学显著变化。
癌前病变中mtDNA含量和柠檬酸合酶活性增加可能是从增生发展为癌的潜在分子标志物。
