Graduate School Biotechnology, Korea University, Sungbuk-Ku, Seoul, South Korea.
J Microencapsul. 2009 May;26(3):235-42. doi: 10.1080/02652040802273469.
A new nutraceutical carrier, chitosan-coated nano-size liposome, which is itself bioactive, is made from phosphatidylcholine (pc) and cholesterol by the sonication method. Different factors related to the size of the liposomes such as the ratio of the pc and cholesterol and the time of sonication are investigated. At the 40 : 60 ratios of pc and cholesterol with 12 min sonication, the smallest liposomes, with mean diameter ∼82 nm, were obtained. Incorporation of chemically labile active ingredients into the chitosan-coated liposomes prevented chemical degradation, which is shown for vitamin E (VE). After VE was loaded to the liposomes, the size of chitosan-coated liposomes increased to 144 nm. The loading efficiency and payload of VE were investigated by loading different amounts of VE to the liposomes which were prepared with different ratios of pc and cholesterol. Chitosan-coated nano-coated nano-size liposomes appeared to be promising VE carriers, with highest loading efficiency over 99% and payload over 27%. The stability of VE-loaded liposomes suspension during the 8 weeks storage is over 90% under 4°C.
一种新的营养药物载体——壳聚糖包裹的纳米脂质体，由 sonication 方法用磷脂酰胆碱（PC）和胆固醇制成，本身具有生物活性。研究了与脂质体大小相关的不同因素，如 PC 和胆固醇的比例以及 sonication 的时间。在 PC 和胆固醇的 40：60 比例下进行 12 分钟的 sonication，可得到最小的脂质体，平均直径约为 82nm。将不稳定的活性成分化学掺入壳聚糖包裹的脂质体中可以防止化学降解，这在维生素 E（VE）中得到了证明。将 VE 加载到脂质体后，壳聚糖包裹的脂质体的粒径增加到 144nm。通过将不同量的 VE 加载到用不同比例的 PC 和胆固醇制备的脂质体中，研究了 VE 的载药量和载药量。壳聚糖包裹的纳米脂质体似乎是一种很有前途的 VE 载体，载药量超过 27%，载药效率超过 99%。在 4°C 下储存 8 周时，VE 负载的脂质体悬浮液的稳定性超过 90%。
