Takamura H, Kito M
Research Institute for Food Science, Kyoto University.
J Biochem. 1991 Mar;109(3):436-9. doi: 10.1093/oxfordjournals.jbchem.a123399.
A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase HPLC using a fluorescence detector. All the molecular species of the derivatives had the same peak area per mole, and the peak areas were proportional to the amounts of the derivatives. Quantification could be carried out at the femtomole level.
开发了一种用于磷脂分子种类定量分析的高灵敏度方法。用磷脂酶C从磷脂制备的二酰基甘油被转化为蒽酰 - 二酰基甘油衍生物，这些衍生物可分离成分子种类，并使用荧光检测器通过反相高效液相色谱法进行灵敏定量。衍生物的所有分子种类每摩尔具有相同的峰面积，且峰面积与衍生物的量成正比。定量可在飞摩尔水平进行。
