Kito M, Takamura H, Narita H, Urade R
J Biochem. 1985 Aug;98(2):327-31. doi: 10.1093/oxfordjournals.jbchem.a135285.
A simple and sensitive method was developed for quantitative analysis of phospholipid molecular species. Diacylglycerols were prepared from phospholipids by phospholipase C treatment and converted to the corresponding dinitrobenzoyl derivatives, which could be sensitively detected at 254 nm. The derivatives of 21 molecular species were resolved by high-performance liquid chromatography with an octadecylsilyl reversed-phase column. All the derivatives had the same peak area per mol, and peak areas were proportional to the amounts of the derivatives. Quantification was carried out at the picomole level.
开发了一种用于磷脂分子种类定量分析的简单且灵敏的方法。通过磷脂酶C处理从磷脂制备二酰基甘油，并将其转化为相应的二硝基苯甲酰衍生物，该衍生物可在254nm处被灵敏检测。21种分子种类的衍生物通过使用十八烷基硅烷反相柱的高效液相色谱法进行分离。所有衍生物每摩尔具有相同的峰面积，且峰面积与衍生物的量成正比。定量在皮摩尔水平进行。
