Yu Marcella, Ng Benny C, Rome Leonard H, Tolbert Sarah H, Monbouquette Harold G
Chemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, California 90095, USA.
Nano Lett. 2008 Oct;8(10):3510-5. doi: 10.1021/nl080536z. Epub 2008 Sep 20.
Vaults are ubiquitous, self-assembled protein nanocapsules with dimension in the sub-100 nm range that are conserved across diverse phyla from worms to humans. Their normal presence in humans at a copy number of over 10,000/cell makes them attractive as potential drug delivery vehicles. Toward this goal, bifunctional amine-reactive reagents are shown to be useful for the reversible cross-linking of recombinant vaults such that they may be closed and opened in a controllable manner.
穹窿体是普遍存在的、自组装的蛋白质纳米胶囊，尺寸在100纳米以下，从蠕虫到人类的各种生物门类中都保守存在。它们在人体中正常存在，每个细胞的拷贝数超过10000个，这使其作为潜在的药物递送载体具有吸引力。为了实现这一目标，双功能胺反应试剂被证明可用于重组穹窿体的可逆交联，从而使其能够以可控的方式关闭和打开。
