Sundin Mikael, Lindblom Anna, Örvell Claes, Barrett A John, Sundberg Berit, Watz Emma, Wikman Agneta, Broliden Kristina, Le Blanc Katarina
Division of Clinical Immunology and Transfusion Medicine, Karolinska Institutet, Stockholm, Sweden.
Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden.
Biol Blood Marrow Transplant. 2008 Oct;14(10):1172-1179. doi: 10.1016/j.bbmt.2008.08.003.
Multipotent mesenchymal stromal cells (MSCs) are used to improve the outcome of hematopoietic stem cell transplantation (HCST) and in regenerative medicine. MSCs may harbor persistent viruses that may compromise their clinical benefit, however. Retrospectively screened, 1 of 20 MSCs from healthy donors contained parvovirus B19 (B19) DNA. MSCs express the B19 receptor (P antigen/globoside) and a co-receptor (Ku 80) and can transmit B19 to bone marrow cells in vitro, suggesting that the virus can persist in the marrow stroma of healthy individuals. Two patients undergoing HSCT received the B19-positive MSCs as treatment for graft-versus-host disease; neither developed viremia nor symptomatic B19 infection. These findings demonstrate for the first time that persistent B19 in MSCs can infect hematopoietic stem cells and underscore the importance of monitoring B19 transmission by MSC products.
多能间充质基质细胞(MSCs)被用于改善造血干细胞移植(HCST)的效果以及再生医学中。然而,MSCs可能携带持续性病毒,这可能会损害其临床益处。经回顾性筛查,20例来自健康供体的MSCs中有1例含有细小病毒B19(B19)DNA。MSCs表达B19受体(P抗原/糖苷脂)和一种共受体(Ku 80),并且能够在体外将B19传播至骨髓细胞,这表明该病毒能够在健康个体的骨髓基质中持续存在。两名接受造血干细胞移植的患者接受了B19阳性的MSCs作为移植物抗宿主病的治疗;两人均未发生病毒血症,也未出现有症状的B19感染。这些发现首次证明MSCs中持续性的B19能够感染造血干细胞,并强调了监测MSC产品传播B19的重要性。