Panyutich A V, Voitenok N N, Lehrer R I, Ganz T
Will Rogers Pulmonary Research Laboratory, School of Medicine, University of California, Los Angeles 90024-1736.
J Immunol Methods. 1991 Aug 9;141(2):149-55. doi: 10.1016/0022-1759(91)90141-2.
We developed and optimized an enzyme immunoassay for human neutrophil defensins, cationic cysteine-rich peptides that participate in host defense and inflammation. The assay utilizes a sandwich design with a monoclonal capture antibody and a biotinylated monoclonal detecting antibody. Cetrimonium bromide is employed to obviate non-specific binding of defensins to surfaces. The assay has a sensitivity of 0.04-0.05 ng/ml and a working range of 0.05-10 ng/ml.
我们开发并优化了一种用于检测人中性粒细胞防御素的酶免疫测定法，中性粒细胞防御素是一类富含半胱氨酸的阳离子肽，参与宿主防御和炎症反应。该测定法采用夹心设计，使用单克隆捕获抗体和生物素化单克隆检测抗体。采用溴化十六烷基三甲基铵来避免防御素与表面的非特异性结合。该测定法的灵敏度为0.04 - 0.05 ng/ml，工作范围为0.05 - 10 ng/ml。
