Barefoot Brice, Thornburg Natalie J, Barouch Daniel H, Yu Jae-Sung, Sample Christopher, Johnston Robert E, Liao Hua Xin, Kepler Thomas B, Haynes Barton F, Ramsburg Elizabeth
Duke Human Vaccine Institute, Duke University School of Medicine, DU Medical Center, 102 Research Drive, Durham, NC 27710, USA.
Vaccine. 2008 Nov 11;26(48):6108-18. doi: 10.1016/j.vaccine.2008.09.007. Epub 2008 Sep 20.
The prevention of infectious disease via prophylactic immunization is a mainstay of global public health efforts. Vaccine design would be facilitated by a better understanding of the type and durability of immune responses generated by different vaccine vectors. We report here the results of a comparative immunogenicity trial of six different vaccine vectors expressing the same insert antigen, cowpox virus B5 (CPXV-B5). Of those vectors tested, recombinant adenovirus (rAd5) was the most immunogenic, inducing the highest titer anti-B5 antibodies and conferring protection from sublethal vaccinia virus challenge in mice after a single immunization. We tested select heterologous prime-boost combinations and identified recombinant vesicular stomatitis virus (rVSV) and recombinant Venezuelan equine encephalitis virus replicons (VRP) as the most synergistic regimen. Comparative data such as those presented here are critical to efforts to generate protective vaccines for emerging infectious diseases as well as for biothreat agents.
通过预防性免疫接种预防传染病是全球公共卫生工作的支柱。更好地了解不同疫苗载体产生的免疫反应的类型和持久性将有助于疫苗设计。我们在此报告了一项比较免疫原性试验的结果，该试验针对六种表达相同插入抗原牛痘病毒B5（CPXV-B5）的不同疫苗载体。在测试的那些载体中，重组腺病毒（rAd5）免疫原性最强，单次免疫后在小鼠中诱导出最高滴度的抗B5抗体，并对亚致死性痘苗病毒攻击提供保护。我们测试了选定的异源初免-加强组合，并确定重组水疱性口炎病毒（rVSV）和重组委内瑞拉马脑炎病毒复制子（VRP）为最具协同作用的方案。此处提供的此类比较数据对于研发针对新出现传染病以及生物威胁制剂的保护性疫苗至关重要。
