通过光开关荧光探针的纳米级定位实现超分辨率显微镜技术
Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes.
作者信息
Bates Mark, Huang Bo, Zhuang Xiaowei
机构信息
School of Engineering and Applied Sciences, Cambridge, MA 02138, United States, USA.
出版信息
Curr Opin Chem Biol. 2008 Oct;12(5):505-14. doi: 10.1016/j.cbpa.2008.08.008.
Abstract
A new form of super-resolution fluorescence microscopy has emerged in recent years, based on the high accuracy localization of individual photo-switchable fluorescent labels. Image resolution as high as 20 nm in the lateral dimensions and 50 nm in the axial direction has been attained with this concept, representing an order of magnitude improvement over the diffraction limit. The demonstration of multicolor imaging with molecular specificity, three-dimensional (3D) imaging of cellular structures, and time-resolved imaging of living cells further illustrates the exciting potential of this method for biological imaging at the nanoscopic scale.
摘要
近年来，基于单个光开关荧光标记的高精度定位，一种新型的超分辨率荧光显微镜应运而生。基于这一概念，在横向维度上已实现高达20纳米的图像分辨率，在轴向方向上则为50纳米，这比衍射极限提高了一个数量级。具有分子特异性的多色成像、细胞结构的三维（3D）成像以及活细胞的时间分辨成像的展示，进一步说明了这种方法在纳米尺度生物成像方面令人兴奋的潜力。
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