利用光激活定位显微镜对单分子轨迹进行高密度映射。

High-density mapping of single-molecule trajectories with photoactivated localization microscopy.

作者信息

Manley Suliana, Gillette Jennifer M, Patterson George H, Shroff Hari, Hess Harald F, Betzig Eric, Lippincott-Schwartz Jennifer

机构信息

National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Nat Methods. 2008 Feb;5(2):155-7. doi: 10.1038/nmeth.1176. Epub 2008 Jan 13.

DOI:10.1038/nmeth.1176

PMID:18193054

Abstract

We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

摘要

我们将光激活定位显微镜（PALM）与活细胞单粒子追踪相结合，创建了一种名为sptPALM的新方法。通过对膜蛋白Gag和VSVG进行成像，我们创建了单分子运动的空间分辨图谱，并且每个细胞获得的轨迹数量比传统单粒子追踪所能实现的多几个数量级。通过探测不同的分子子集，sptPALM可以深入了解膜中空间和时间异质性的起源。