De Cesare A, Parisi A, Bondioli V, Normanno G, Manfreda G
Department of Food Science, Alma Mater Studiorum-University of Bologna, Via del Florio 2, 40064 Ozzano dell'Emilia (BO), Italy.
Poult Sci. 2008 Oct;87(10):2152-9. doi: 10.3382/ps.2007-00441.
The main aim of this study was to trace Campylobacter subtypes colonizing Italian broilers and carcasses in and between flocks. Overall, 209 Campylobacter isolates were collected from ceca (n = 94) and carcasses (n = 115) of broilers belonging to 3 different flocks reared in the same farm during subsequent rotations and processed in the same slaughterhouse. All isolates were identified by multiplex polymerase chain reaction and genotyped by amplified fragment length polymorphism. Furthermore, 166 out of 209 strains were phenotyped by antimicrobial resistance profile (R-type). The results of genetic and phenotypic characterization showed that (1) multiple Campylobacter species and subtypes can colonize the same broiler and carcass; (2) common Campylobacter subtypes in ceca and carcasses seem to be rare; and (3) carryover of Campylobacter subtypes between broiler flocks in the same house rarely occurs. The outcomes of this study should be taken into account for setting of isolate collection during epidemiological investigations to check sources and transmission routs of Campylobacter in broilers and poultry products.
本研究的主要目的是追踪在鸡群内和鸡群间定植于意大利肉鸡及屠体的弯曲杆菌亚型。总体而言，从同一养殖场后续轮次饲养并在同一屠宰场加工的3个不同鸡群的肉鸡盲肠（n = 94）和屠体（n = 115）中收集了209株弯曲杆菌分离株。所有分离株均通过多重聚合酶链反应进行鉴定，并通过扩增片段长度多态性进行基因分型。此外，209株菌株中的166株通过抗菌药物耐药谱（R型）进行表型分析。遗传和表型特征分析结果表明：（1）多种弯曲杆菌菌种和亚型可定植于同一肉鸡及屠体；（2）盲肠和屠体中常见的弯曲杆菌亚型似乎很少见；（3）同一鸡舍内肉鸡群之间弯曲杆菌亚型的携带情况很少发生。在开展流行病学调查以检查肉鸡和禽肉产品中弯曲杆菌的来源和传播途径时，应考虑本研究结果以确定分离株的收集工作。
