Delmi M, Vaudaux P, Lew D P, Vasey H
Department of Surgery, University Hospital, Geneva, Switzerland.
J Orthop Res. 1994 May;12(3):432-8. doi: 10.1002/jor.1100120316.
Staphylococcal infection of various prosthetic and internal fixation devices is a major complication associated with orthopaedic surgery. This study investigated the role of the host protein fibronectin in promoting adhesion of Staphylococcus aureus and Staphylococcus epidermidis to metallic surfaces representing materials used for orthopaedic devices. Pure human fibronectin was adsorbed in vitro onto coverslips (0.8 x 0.8 cm) of stainless steel, pure titanium, or titanium-aluminum-niobium alloy. In vitro bacterial adhesion was promoted more strongly by the metallic surfaces coated with fibronectin than by albumin-coated controls for two strains of S. aureus and one strain of S. epidermidis. Furthermore, with the fibronectin-coated coverslips, bacterial adhesion to titanium alloy was significantly greater than adhesion to stainless steel. Adhesion of the three staphylococcal strains was promoted more strongly by coverslips explanted from the subcutaneous space of guinea pigs and tested under similar conditions than by albumin-coated controls. Incubation of either in vitro fibronectin-coated or explanted metallic coverslips with anti-fibronectin antibodies produced a significant decrease in staphylococcal adhesion. These results suggest that the presence of fibronectin on the surface of implanted metallic devices is an important determinant of colonization of orthopaedic biomaterials by staphylococci.
各种假体和内固定装置的葡萄球菌感染是骨科手术相关的主要并发症。本研究调查了宿主蛋白纤连蛋白在促进金黄色葡萄球菌和表皮葡萄球菌黏附于代表骨科装置所用材料的金属表面方面的作用。将纯人纤连蛋白体外吸附到不锈钢、纯钛或钛铝铌合金的盖玻片（0.8×0.8厘米）上。对于两株金黄色葡萄球菌和一株表皮葡萄球菌，与白蛋白包被的对照相比，纤连蛋白包被的金属表面对体外细菌黏附的促进作用更强。此外，对于纤连蛋白包被的盖玻片，细菌对钛合金的黏附显著大于对不锈钢的黏附。与白蛋白包被的对照相比，从豚鼠皮下空间取出并在类似条件下测试的盖玻片对三种葡萄球菌菌株黏附的促进作用更强。用抗纤连蛋白抗体孵育体外纤连蛋白包被的或取出的金属盖玻片，会使葡萄球菌黏附显著减少。这些结果表明，植入金属装置表面存在纤连蛋白是葡萄球菌在骨科生物材料上定植的重要决定因素。
