Liu Xiong, Lloyd Mark C, Fedorenko Inna V, Bapat Priya, Zhukov Tatyana, Huo Qun
Nanoscience Technology Center, Department of Chemistry, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA.
Nanomedicine (Lond). 2008 Oct;3(5):617-26. doi: 10.2217/17435889.3.5.617.
BACKGROUND & AIMS: Gold nanoparticles are excellent photon-thermal energy converters. The purpose of this work was to investigate the influence of gold nanoparticles on the photothermalysis of A549 lung tumor cells.
MATERIALS & METHODS: A549 lung tumor cells were exposed to goat antihuman immunoglobulin (Ig)G-conjugated gold nanospheres (40 nm) and were then imaged under a dark-field microscope. The live cells were then subjected to photoirradiation using a 633-nm laser at different power levels. The viability of tumor cells under laser irradiation was monitored by confocal microscopy using a viability-assay kit.
RESULTS & DISCUSSION: The death rates of A549 lung tumor cells after gold nanoparticle exposure increased significantly under laser irradiation. The maximum initial cell death rate was observed at a laser power level of 3.75 mW, with the initial deactivation rate accelerated by a factor of 6.6 and a total loss of 92% of cell viability.
This work demonstrated potential applications of gold nanospheres as both imaging probes and enhancing agents for photothermal therapy of cancer.
金纳米颗粒是出色的光-热能转换器。本研究旨在探讨金纳米颗粒对A549肺肿瘤细胞光热分解的影响。
将A549肺肿瘤细胞暴露于山羊抗人免疫球蛋白（Ig）G偶联的金纳米球（40纳米），然后在暗场显微镜下成像。随后，使用633纳米激光以不同功率水平对活细胞进行光照射。使用活力检测试剂盒通过共聚焦显微镜监测激光照射下肿瘤细胞的活力。
在激光照射下，金纳米颗粒暴露后A549肺肿瘤细胞的死亡率显著增加。在激光功率水平为3.75毫瓦时观察到最大初始细胞死亡率，初始失活率加快了6.6倍，细胞活力总损失达92%。
本研究证明了金纳米球作为癌症光热治疗的成像探针和增强剂的潜在应用。
