Cândido Teresinha Cristina, Perri Sílvia Helena Venturoli, Gerzoschkwitz Tatiana de Oliveira, Luvizotto Maria Cecília Rui, de Lima Valéria Marçal Felix
Departamento de Clinica, Cirurgia e Reprodução Animal, Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, SP, Brazil.
Vet Parasitol. 2008 Nov 7;157(3-4):175-81. doi: 10.1016/j.vetpar.2008.08.010. Epub 2008 Aug 22.
This study evaluated the performance of crude total antigen (CTA) and fucose-mannose ligand antigen (FML) in an enzyme-linked immunosorbent assay for diagnosis of canine visceral leishmaniasis (CVL). The assays used sera from known negative controls (n=30), clinically symptomatic (n=30) and oligosymptomatic (n=30) parasitologically proven infection (by microscopy). Aspirates of popliteal lymph node from infected canines were colleted to score parasitism and compared with the ELISA results. The study indicated that FML used in ELISA provided high sensitivity for detecting oligosymptomatic dogs (90%) and CTA showed greater sensitivity than FML for symptomatic canines (90%). In oligosymptomatic dogs, specificity was 100% for CTA-ELISA, but in symptomatic dogs, FML specificity was higher (96.7%) than CTA-ELISA (93.3%). A significant correlation was observed between the degree of parasitism and the results obtained in CTA-ELISA. Since no available antigen offers 100% specificity and sensitivity for CVL diagnosis, the choice of antigen used must depend on the aim of the investigation.
本研究评估了粗制总抗原（CTA）和岩藻糖-甘露糖配体抗原（FML）在酶联免疫吸附测定法中用于诊断犬内脏利什曼病（CVL）的性能。该测定使用了来自已知阴性对照（n = 30）、临床有症状（n = 30）和症状轻微（n = 30）且经寄生虫学证实感染（通过显微镜检查）的犬的血清。采集感染犬的腘淋巴结抽吸物以评估寄生虫感染情况，并与酶联免疫吸附测定结果进行比较。研究表明，酶联免疫吸附测定中使用的FML对检测症状轻微的犬具有高灵敏度（90%），而CTA对有症状的犬显示出比FML更高的灵敏度（90%）。在症状轻微的犬中，CTA-酶联免疫吸附测定的特异性为100%，但在有症状的犬中，FML的特异性（96.7%）高于CTA-酶联免疫吸附测定（93.3%）。在CTA-酶联免疫吸附测定中观察到寄生虫感染程度与所得结果之间存在显著相关性。由于没有可用抗原对CVL诊断具有100%的特异性和灵敏度，所用抗原的选择必须取决于研究目的。
