Gonzalo-Ruiz Javier, Mas Roser, de Haro Carmen, Cabruja Enric, Camero Rafael, Alonso-Lomillo M Asuncion, Muñoz F Javier
Centro Nacional de Microelectrónica (IMB-CNM), CSIC, Esfera UAB, Campus Universidad Autónoma de Barcelona, Bellaterra, Barcelona, Spain.
Biosens Bioelectron. 2009 Feb 15;24(6):1788-91. doi: 10.1016/j.bios.2008.07.051. Epub 2008 Aug 3.
A novel and rapid approach to quantify chloride concentration in sweat for early detection of cystic fibrosis (CF) is shown in this work. Disposable screen-printed sensor (SPS) devices capable to induce sweat and measure the chloride concentration are presented. Pilocarpine, which was forced into de skin by means of iontophoresis, has been used to stimulate the sweat glands. Chloride concentration has been directly measured on the skin by potentiometry. The performance of the devices has been tested in synthetic samples, obtaining good agreement with the Nernst equation. Sensors reproducibility has been analyzed in terms of residual standard deviation (RSD), obtaining a value of 8% (n=6 and alpha=0.05). Finally, the application of these sensors in several volunteers has been carried out. The results were compared with the method generally used in hospitals, obtaining deviations minor than 8%.
本文展示了一种用于早期检测囊性纤维化（CF）的新颖且快速的汗液氯离子浓度定量方法。介绍了能够诱导出汗并测量氯离子浓度的一次性丝网印刷传感器（SPS）设备。通过离子电渗疗法将毛果芸香碱导入皮肤，用于刺激汗腺。通过电位分析法直接在皮肤上测量氯离子浓度。该设备的性能已在合成样品中进行测试，与能斯特方程取得了良好的一致性。已根据残余标准偏差（RSD）分析了传感器的重现性，得到的值为8%（n = 6且α = 0.05）。最后，在多名志愿者身上进行了这些传感器的应用。将结果与医院普遍使用的方法进行比较，得到的偏差小于8%。
