Department of Botany, Panjab University, Chandigarh, 160014, India.
Appl Biochem Biotechnol. 2024 Jan;196(1):332-349. doi: 10.1007/s12010-023-04498-7. Epub 2023 May 2.
Endophytic fungi live symbiotically inside plants and are hidden source of natural bioactive molecules. The present study was carried out to investigate the phytochemical analysis and antioxidant activity of endophytic fungi isolated from the ethnomedicinal plant Dillenia indica L. The ethyl acetate crude extracts of the endophytic fungal strains were preliminarily evaluated for their phytochemical analysis, and the results showed the presence of alkaloids, flavonoids, phenolics, terpene, and saponins. The crude extracts of more than 60% of the isolates showed 50-90% antioxidant activity by DPPH and HO assay. The inhibition percentage of ethyl acetate extracts ranges from 34.05 to 91.5%, whereas IC values vary from 72.2 to 691.14%. Among all the strains, Fomitopsis meliae crude extract showed a maximum inhibition percentage, i.e., 91.5%, with an IC value of 88.27 µg/mL. Chaetomium globosum showed significant activity having an inhibition percentage of 89.88% and an IC value of 74.44 µg/mL. The total phenolic and flavonoid content in the crude extract of Chaetomium globosum was 37.4 mg gallic acid equivalent (GAE)/g DW and 31.0 mg quercetin equivalent (GAE)/g DW. GC-MS analysis of crude extract of C. globosum revealed different compounds, such as squalene; butanoic acid, 2-methyl-; hexadecanoic acid; 2-propanone, 1-phenyl-; 5-oxo-pyrrolidine-2-carboxylic acid methyl ester; 9,12-octadecadienoic acid (z)- etc. Many of these belong to phenolics, which are natural antioxidant compounds. The findings suggested that endophytic fungi associated with Dillenia indica L. can be a potential source of novel antioxidant compounds.
内生真菌与植物共生，并隐藏着天然生物活性分子的来源。本研究旨在从药用植物杜英（Dillenia indica L.）中分离内生真菌，对其进行植物化学分析和抗氧化活性研究。初步评价了内生真菌菌株的乙酸乙酯粗提取物的植物化学分析，结果表明存在生物碱、黄酮类、酚类、萜类和皂苷。超过 60%的分离物的粗提取物在 DPPH 和 HO 测定中显示出 50-90%的抗氧化活性。乙酸乙酯提取物的抑制率范围为 34.05%至 91.5%，而 IC 值范围为 72.2 至 691.14%。在所有菌株中，Meliaceae 内生真菌粗提取物表现出最大的抑制百分比，即 91.5%，IC 值为 88.27 µg/mL。Chaetomium globosum 表现出显著的活性，抑制百分比为 89.88%，IC 值为 74.44 µg/mL。Chaetomium globosum 粗提取物中的总酚和类黄酮含量分别为 37.4 mg 没食子酸当量（GAE）/g DW 和 31.0 mg 槲皮素当量（GAE）/g DW。GC-MS 分析表明，Globosum 粗提取物中含有不同的化合物，如角鲨烯；2-甲基丁酸；十六烷酸；1-苯-2-丙酮；5-氧代-吡咯烷-2-羧酸甲酯；9,12-十八碳二烯酸（Z）等。其中许多属于酚类化合物，是天然抗氧化剂。研究结果表明，与杜英相关的内生真菌可能是新型抗氧化化合物的潜在来源。
