Department of Pharmacology, TIFAC CORE in Herbal Drugs, JSS College of Pharmacy, Ootacamund 643001 and Green Milk Concepts, Herbal Division of Apex Laboratories, Chennai 600038, India.
Evid Based Complement Alternat Med. 2008 Sep;5(3):289-94. doi: 10.1093/ecam/nem034.
Herbex-kid (HK), a polyherbal formulation was evaluated in various experimental allergic models of Type I hypersensitivity reactions. Compound 48/80 (C 48/80) has been shown to induce rat mesentery mast cell degranulation and HK (1.07, 10.75 and 107.5 mg ml(-1)) inhibited the mast cell degranulation in a dose dependent manner. HK (1.07, 10.75 and 107.5 mg kg(-1); p.o.) showed dose-dependent protection against C 48/80 induced systemic anaphylaxis in male Balb/C mice. In active anaphylaxis model, male Wistar rats orally administered with 10.75 and 107.5 mg kg(-1) of HK showed significant (P < 0.01) protection against mast cell degranulation, while in passive anaphylaxis model, only at 107.5 mg kg(-1) showed significant (P < 0.01) reduction in mast cell degranulation. HK at all dose levels was able to significantly decrease the time spent in nasal rubbing in Wistar rats sensitized to ovalbumin, while only at 107.5 mg kg(-1) it showed significant (P < 0.01) reduction in number of sneezes. In C 48/80-induced skin itch model, all dose levels of HK significantly (P < 0.001) decreased the time spent in itching and the number of itches. HK did not produce any significant inhibition in histamine induced contraction in guinea pig ileum. From the above findings we conclude that the HK possesses antiallergic activity mediated by reducing of the release mediators from mast cells and also by 5-HT antagonism without the involvement of histamine (H1) receptors.
Herbex-kid (HK)，一种复方草药制剂，已在 I 型超敏反应的各种实验性过敏模型中进行了评估。化合物 48/80（C 48/80）已被证明可诱导大鼠肠系膜肥大细胞脱颗粒，而 HK（1.07、10.75 和 107.5 mg/ml）可呈剂量依赖性抑制肥大细胞脱颗粒。HK（1.07、10.75 和 107.5 mg/kg；po）对雄性 Balb/C 小鼠的 C 48/80 诱导的全身性过敏反应具有剂量依赖性保护作用。在主动过敏模型中，雄性 Wistar 大鼠口服 10.75 和 107.5 mg/kg 的 HK 可显著（P < 0.01）保护肥大细胞脱颗粒，而在被动过敏模型中，仅在 107.5 mg/kg 时可显著（P < 0.01）减少肥大细胞脱颗粒。HK 在所有剂量水平均可显著减少卵清蛋白致敏的 Wistar 大鼠的鼻擦时间，而仅在 107.5 mg/kg 时可显著（P < 0.01）减少打喷嚏次数。在 C 48/80 诱导的皮肤瘙痒模型中，HK 的所有剂量水平均可显著（P < 0.001）减少瘙痒时间和搔抓次数。HK 对豚鼠回肠中组胺诱导的收缩没有产生任何显著抑制作用。从上述发现中，我们得出结论，HK 通过减少肥大细胞释放介质并通过 5-HT 拮抗作用（不涉及组胺（H1）受体）发挥抗过敏活性。
