Towns J K, Regnier F E
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
Anal Chem. 1991 Jun 1;63(11):1126-32. doi: 10.1021/ac00011a013.
Capillary zone electrophoretic separations of proteins have been achieved by using nonionic surfactant coated capillaries. Capillaries were prepared by derivatization of the silica surface with octadecylsilane followed by the deposition of a layer of nonionic surfactant from an aqueous solution above the critical micelle concentration. This coating is of sufficient thickness and hydrophilicity to reduce both protein adsorption and electroosmotic pumping. This hydrophilic coating reduces electroosmotic pumping 5-8-fold while resolving proteins quickly and efficiently with good recovery. The coating provides a stable and reproducible means of deactivation, while the rate of electroosmotic pumping stays relatively constant throughout the pH range 4-11. This allows the pH to be varied to enhance selectivity without adversely affecting the flow rate.
通过使用非离子表面活性剂涂层毛细管实现了蛋白质的毛细管区带电泳分离。毛细管的制备方法是先用十八烷基硅烷对二氧化硅表面进行衍生化，然后从高于临界胶束浓度的水溶液中沉积一层非离子表面活性剂。这种涂层具有足够的厚度和亲水性，可减少蛋白质吸附和电渗泵送。这种亲水性涂层可将电渗泵送降低5至8倍，同时能快速、高效地分离蛋白质，回收率良好。该涂层提供了一种稳定且可重复的去活化方法，而在4至11的pH范围内，电渗泵送速率保持相对恒定。这使得可以改变pH值来提高选择性，而不会对流速产生不利影响。
