Bujia J, Wilmes E, Krombach F, Hammer C, Kastenbauer E
Department of Otorhinolaryngology, Ludwig-Maximilians University, Munich, Germany.
Am J Otolaryngol. 1990 Sep-Oct;11(5):339-44. doi: 10.1016/0196-0709(90)90065-4.
The presence and distribution of class II antigens on human nasal cartilage was investigated using monoclonal antibodies directed against HLA-DR, HLA-DP, and HLA-DQ molecules. Tissue sections and chondrocytes in suspension were prepared for immunoperoxidase staining and flow cytometric analysis. Class II antigens were detected primarily on the perichondrium. In contrast, chondrocytes, either isolated or in tissue sections, were completely negative, suggesting that cells of the perichondrium, may, by themselves, be capable of initiating a rejection process.
利用针对HLA - DR、HLA - DP和HLA - DQ分子的单克隆抗体，研究了人类鼻软骨上II类抗原的存在和分布情况。制备了组织切片和悬浮的软骨细胞用于免疫过氧化物酶染色和流式细胞术分析。II类抗原主要在软骨膜上检测到。相比之下，无论是分离的软骨细胞还是组织切片中的软骨细胞均呈完全阴性，这表明软骨膜细胞自身可能有能力启动排斥反应过程。
