Cox J C, Drane D P, Jones S L, Ridge S, Milner A R
Commonwealth Serum Laboratories, Parkville, Victoria.
Aust Vet J. 1991 May;68(5):157-60. doi: 10.1111/j.1751-0813.1991.tb03167.x.
An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.
开发了一种用于牛副结核病的吸收酶免疫测定（EIA）试验，其中交叉反应抗体的吸收在溶液中作为快速反应发生，而不是与完整生物体过夜反应并随后进行离心步骤。总测试时间减少到不到2小时，操作最少。该试验在澳大利亚牛副结核病流行地区和无牛副结核病地区的牛群中进行了评估。特异性为99.8%。敏感性计算受被测牛群病史的影响。然而，EIA在临床疾病发作前检测出超过80%的动物，并且在首次检测到其粪便中的副结核分枝杆菌时或之前，65%的粪便排菌者EIA呈阳性。该试验应有助于流行病学研究，并成为牛副结核病管理和控制的有用工具。
