Milner A R, Mack W N, Coates K J, Hill J, Gill I, Sheldrick P
Molecular Biology Unit, Department of Agriculture and Rural Affairs, Parkville, Vic., Australia.
Vet Microbiol. 1990 Nov;25(2-3):193-8. doi: 10.1016/0378-1135(90)90076-8.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Mycobacterium paratuberculosis in cattle was evaluated in three herds known to have Johne's disease. Prior to testing, the plasma was absorbed with dried Mycobacterium phlei in order to remove cross-reacting antibody specificities. The sensitivity and specificity of the ELISA were calculated after repeatedly testing 327 cattle in the infected herds. Of these, 53 animals had one or more positive faecal cultures or had post-mortem histopathological evidence of infection. The other 274 had three or more negative faecal culture results, and were regarded as non-infected for the purpose of evaluation of the ELISA. Using these criteria for the presence or absence of infection, the M. phlei-absorbed ELISA under field conditions had a 57% sensitivity and a 98.9% specificity. The sensitivity of the absorbed ELISA depended on the stage of disease of the animal under test. In general, it appeared that animals in the more advanced stages of disease were absorbed ELISA positive, whereas those in the early stages of infection were not detected. These results indicate that the M. phlei-absorbed ELISA has an important role as a test for the diagnosis and control of Johne's disease in cattle.
在三个已知患有副结核的牛群中，对一种用于检测牛抗副结核分枝杆菌抗体的酶联免疫吸附测定（ELISA）进行了评估。在检测之前，血浆用干燥的草分枝杆菌进行吸附，以去除交叉反应抗体特异性。在对感染牛群中的327头牛进行反复检测后，计算了ELISA的敏感性和特异性。其中，53头动物有一次或多次粪便培养阳性，或有死后组织病理学感染证据。另外274头有三次或更多次粪便培养阴性结果，在评估ELISA时被视为未感染。使用这些感染存在与否的标准，在现场条件下，经草分枝杆菌吸附的ELISA敏感性为57%，特异性为98.9%。吸附ELISA的敏感性取决于受试动物的疾病阶段。一般来说，似乎处于疾病较晚期的动物经吸附ELISA检测为阳性，而处于感染早期的动物则未被检测到。这些结果表明，经草分枝杆菌吸附的ELISA在牛副结核的诊断和控制检测中具有重要作用。
