Peterson G, Barnes S
Department of Biochemistry, University of Alabama, Birmingham 35294.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):661-7. doi: 10.1016/0006-291x(91)91423-a.
The effect of isoflavones on the growth of the human breast carcinoma cell lines, MDA-468 (estrogen receptor negative), and MCF-7 and MCF-7-D-40 (estrogen receptor positive), has been examined. Genistein is a potent inhibitor of the growth of each cell line (IC50 values from 6.5 to 12.0 micrograms/ml), whereas biochanin A and daidzein are weaker growth inhibitors (IC50 values from 20 to 34 micrograms/ml). The isoflavone beta-glucosides, genistin and daidzin, have little effect on growth (IC50 values greater than 100 micrograms/ml). The presence of the estrogen receptor is not required for the isoflavones to inhibit tumor cell growth (MDA-468 vs MCF-7 cells). In addition, the effects of genistein and biochanin A are not attenuated by overexpression of the multi-drug resistance gene product (MCF-7-D40 vs MCF-7 cells).
已研究了异黄酮对人乳腺癌细胞系MDA - 468（雌激素受体阴性）、MCF - 7和MCF - 7 - D - 40（雌激素受体阳性）生长的影响。染料木黄酮是每种细胞系生长的强效抑制剂（IC50值为6.5至12.0微克/毫升），而鹰嘴豆芽素A和大豆苷元是较弱的生长抑制剂（IC50值为20至34微克/毫升）。异黄酮β - 葡萄糖苷，染料木苷和大豆苷对生长几乎没有影响（IC50值大于100微克/毫升）。异黄酮抑制肿瘤细胞生长不需要雌激素受体的存在（MDA - 468与MCF - 7细胞）。此外，多药耐药基因产物的过表达不会减弱染料木黄酮和鹰嘴豆芽素A的作用（MCF - 7 - D40与MCF - 7细胞）。
