Tan Wui Siew, Lewis Christina L, Horelik Nicholas E, Pregibon Daniel C, Doyle Patrick S, Yi Hyunmin
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Langmuir. 2008 Nov 4;24(21):12483-8. doi: 10.1021/la802089q. Epub 2008 Oct 4.
We demonstrate hierarchical assembly of tobacco mosaic virus (TMV)-based nanotemplates with hydrogel-based encoded microparticles via nucleic acid hybridization. TMV nanotemplates possess a highly defined structure and a genetically engineered high density thiol functionality. The encoded microparticles are produced in a high throughput microfluidic device via stop-flow lithography (SFL) and consist of spatially discrete regions containing encoded identity information, an internal control, and capture DNAs. For the hybridization-based assembly, partially disassembled TMVs were programmed with linker DNAs that contain sequences complementary to both the virus 5' end and a selected capture DNA. Fluorescence microscopy, atomic force microscopy (AFM), and confocal microscopy results clearly indicate facile assembly of TMV nanotemplates onto microparticles with high spatial and sequence selectivity. We anticipate that our hybridization-based assembly strategy could be employed to create multifunctional viral-synthetic hybrid materials in a rapid and high-throughput manner. Additionally, we believe that these viral-synthetic hybrid microparticles may find broad applications in high capacity, multiplexed target sensing.
我们通过核酸杂交展示了基于烟草花叶病毒（TMV）的纳米模板与基于水凝胶的编码微粒的分层组装。TMV纳米模板具有高度明确的结构和基因工程高密度硫醇功能。编码微粒通过停流光刻（SFL）在高通量微流控装置中产生，由包含编码身份信息、内部控制和捕获DNA的空间离散区域组成。对于基于杂交的组装，用含有与病毒5'端和选定捕获DNA互补序列的接头DNA对部分拆解的TMV进行编程。荧光显微镜、原子力显微镜（AFM）和共聚焦显微镜结果清楚地表明，TMV纳米模板以高空间和序列选择性轻松组装到微粒上。我们预计，我们基于杂交的组装策略可用于快速、高通量地创建多功能病毒-合成杂化材料。此外，我们相信这些病毒-合成杂化微粒可能在高容量、多重目标传感中找到广泛应用。
