Daniel M R, Burnett H E
J.K. Douglas Cancer Research Laboratories, Clatterbridge Hospital, Bebington, Merseyside, UK.
Int J Exp Pathol. 1991 Aug;72(4):397-405.
The constitutive cell types of the two lung cancer cell lines CaLu1 and CaLu3 have been investigated by immunocytochemical staining with markers for cytokeratins, vimentin, carcinoembryonic antigen and the epithelial cell epitope recognized by the monoclonal antibody Ber-EP4. The cells of both lines reacted with vimentin, with CAM 5.2, which is a marker for cytokeratins 8, 18 and 19, and with a specific marker for cytokeratin 19. CaLu3 cultures showed patchy staining, and CaLu1 none, when treated with a monoclonal antibody reactive with cytokeratins 13, 14 and 17. CaLu3 cells all reacted strongly with Ber-EP4 and the antibody to carcinoembryonic antigen, whereas CaLu1 gave a negative reaction with both these reagents. These results indicate that the CaLu3 line has retained antigens characteristic of some bronchial adenocarcinomata, and that the CaLu1 line was derived from a mesothelioma rather than a pleural metastasis of a squamous carcinoma.
通过使用细胞角蛋白、波形蛋白、癌胚抗原以及单克隆抗体Ber-EP4识别的上皮细胞表位的标志物进行免疫细胞化学染色，对两种肺癌细胞系CaLu1和CaLu3的组成细胞类型进行了研究。两个细胞系的细胞均与波形蛋白、作为细胞角蛋白8、18和19标志物的CAM 5.2以及细胞角蛋白19的特异性标志物发生反应。当用与细胞角蛋白13、14和17反应的单克隆抗体处理时，CaLu3培养物显示出斑片状染色，而CaLu1则无染色。CaLu3细胞均与Ber-EP4和癌胚抗原抗体发生强烈反应，而CaLu1对这两种试剂均呈阴性反应。这些结果表明，CaLu3细胞系保留了一些支气管腺癌的特征性抗原，并且CaLu1细胞系源自间皮瘤而非鳞状细胞癌的胸膜转移瘤。
