Jeon Che Ok, Lim Jee-Min, Jang Ho Hee, Park Dong-Jin, Xu Li-Hua, Jiang Cheng-Lin, Kim Chang-Jin
Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2282-6. doi: 10.1099/ijs.0.65369-0.
Two Gram-positive, motile, rod-shaped, halophilic bacteria showing optimum growth at 5-7 % (w/v) NaCl were isolated from a salt lake, Xin-Jiang Province, China. The strains, designated BH312(T) and BH314, grew in the presence of 1-18 % (w/v) NaCl and at temperatures of 15-50 degrees C (optimum: 40 degrees C) and pH values of 5.5-10.0 (optimum: pH 7.5-8.0). The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The DNA G+C content was 38.8-39.0 mol% and the predominant lipoquinone was MK-7. The major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolates formed a cluster with Gracilibacillus orientalis XH-63(T) within the genus Gracilibacillus. The levels of 16S rRNA gene sequence similarity with closely related type strains were lower than 96.2 %. On the basis of phylogenetic, phenotypic and chemotaxonomic properties, the isolates represent a novel species of the genus Gracilibacillus for which the name Gracilibacillus lacisalsi sp. nov. is proposed. The type strain is BH312(T) (=KCTC 13129(T)=DSM 19029(T)).
从中国新疆的一个盐湖中分离出两株革兰氏阳性、具运动性、杆状的嗜盐细菌，它们在5-7%（w/v）NaCl浓度下生长最佳。这两株菌分别命名为BH312(T)和BH314，能在1-18%（w/v）NaCl存在的条件下生长，生长温度范围为15-50℃（最适温度：40℃），pH值范围为5.5-10.0（最适pH：7.5-8.0）。主要的细胞脂肪酸为anteiso-C(15 : 0)、iso-C(15 : 0)和anteiso-C(17 : 0)。DNA的G+C含量为38.8-39.0 mol%，主要的脂醌为MK-7。主要的细胞磷脂为磷脂酰甘油、二磷脂酰甘油和两种未鉴定的磷脂。基于16S rRNA基因序列的系统发育分析表明，这些分离株与东方纤细芽孢杆菌XH-63(T)在纤细芽孢杆菌属内形成一个聚类。与密切相关的模式菌株的16S rRNA基因序列相似性水平低于96.2%。基于系统发育、表型和化学分类学特性，这些分离株代表了纤细芽孢杆菌属的一个新物种，为此提出新名称纤细盐湖芽孢杆菌（Gracilibacillus lacisalsi sp. nov.）。模式菌株为BH312(T)（=KCTC 13129(T)=DSM 19029(T)）。
