Chen Yi-Guang, Zhang Yu-Qin, Liu Zhu-Xiang, Zhuang Da-Chun, Klenk Hans-Peter, Tang Shu-Kun, Cui Xiao-Long, Li Wen-Jun
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2505-9. doi: 10.1099/ijs.0.010801-0. Epub 2009 Jul 21.
A moderately halophilic, endospore-forming, Gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 078133(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Strain JSM 078133(T) was able to grow with 1-23 % (w/v) total salts (optimum, 5-10 %) and at pH 6.0-10.0 (optimum, pH 7.5) and 10-45 degrees C (optimum, 30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major cellular fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(15 : 0). The genomic DNA G+C content of strain JSM 078133(T) was 42.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078133(T) belongs to the genus Halobacillus, and was related most closely to the type strains of Halobacillus campisalis (97.1 % sequence similarity), Halobacillus alkaliphilus (97.0 %) and Halobacillus yeomjeoni (96.8 %). The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078133(T) represents a novel species of the genus Halobacillus, for which the name Halobacillus salsuginis sp. nov. is proposed. The type strain is JSM 078133(T) (=DSM 21185(T)=KCTC 13236(T)).
从中国湖南省某盐矿采集的一份地下卤水样品中，分离出了一株中度嗜盐、产芽孢、革兰氏阳性、过氧化氢酶和氧化酶阳性、具运动性、杆状、需氧的细菌，命名为菌株JSM 078133(T)。菌株JSM 078133(T)能够在总盐含量为1 - 23%（w/v）（最适为5 - 10%）、pH值为6.0 - 10.0（最适pH值为7.5）以及温度为10 - 45℃（最适温度为30℃）的条件下生长。细胞壁肽聚糖中存在内消旋二氨基庚二酸。主要呼吸醌为甲基萘醌7（MK - 7），主要细胞脂肪酸为anteiso - C(15 : 0)、anteiso - C(17 : 0)和iso - C(15 : 0)。菌株JSM 078133(T)的基因组DNA G + C含量为42.2 mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株JSM 078133(T)属于嗜盐芽孢杆菌属，与Campisalis嗜盐芽孢杆菌（序列相似性为97.1%）、嗜碱嗜盐芽孢杆菌（97.0%）和Yeomjeoni嗜盐芽孢杆菌（96.8%）的模式菌株亲缘关系最为密切。系统发育分析、DNA - DNA相关性数据、表型特征和化学分类数据共同支持了菌株JSM 078133(T)代表嗜盐芽孢杆菌属一个新物种的观点，为此提出新物种名称盐渍嗜盐芽孢杆菌（Halobacillus salsuginis sp. nov.）。模式菌株为JSM 078133(T)（=DSM 21185(T)=KCTC 13236(T)）。
