Kim Yoon-Jin, Cho Si Young, Yun Cheol Hee, Moon Yang Soo, Lee Tae Ryong, Kim Sang Hoon
Department of Biology, Kyung Hee University, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2008 Dec 5;377(1):297-302. doi: 10.1016/j.bbrc.2008.09.129. Epub 2008 Oct 7.
Cidec is a lipid droplet-associated protein, which inhibits lipolysis, leading to the accumulation of triglycerides in adipocytes. However, the transcriptional regulation of Cidec in adipocyte remains unknown. In the present study we investigated that the mouse Cidec transcript is regulated by PPARgamma2. After the differentiation of adipocyte, the expression pattern of Cidec was similar to that of PPARgamma2. In the presence of a PPARgamma agonist, the level of Cidec mRNA was highly increased. In addition, putative PPRE sites were identified in the Cidec promoter. By chromatin immunoprecipitation assay and reporter assay, we observed the binding of PPARgamma2 to the promoter of Cidec. Gel shift assay and the mutagenesis study were showed that the -219/-207 region of the Cidec promoter could function as a PPRE of the Cidec promoter. These results suggest that PPARgamma2 is required for the transcriptional activity of Cidec during adipogenesis, which could be contributed to understand the molecular mechanism of lipid droplet formation in adipocytes.
Cidec是一种与脂滴相关的蛋白质，它抑制脂肪分解，导致甘油三酯在脂肪细胞中积累。然而，Cidec在脂肪细胞中的转录调控仍不清楚。在本研究中，我们研究了小鼠Cidec转录本受PPARγ2调控。脂肪细胞分化后，Cidec的表达模式与PPARγ2相似。在存在PPARγ激动剂的情况下，Cidec mRNA水平显著升高。此外，在Cidec启动子中鉴定出假定的PPRE位点。通过染色质免疫沉淀分析和报告基因分析，我们观察到PPARγ2与Cidec启动子的结合。凝胶迁移分析和诱变研究表明，Cidec启动子的-219/-207区域可作为Cidec启动子的PPRE发挥作用。这些结果表明，PPARγ2在脂肪生成过程中是Cidec转录活性所必需的，这有助于理解脂肪细胞中脂滴形成的分子机制。
