Apple Fred S, Murakami MaryAnn M, Ler Ranka, Walker Dana, York Malcolm
Department of Laboratory Medicine and Pathology, Hennepin County Medical Center and the University of Minnesota School of Medicine, Minneapolis, MN 55415, USA.
Clin Chem. 2008 Dec;54(12):1982-9. doi: 10.1373/clinchem.2007.097568. Epub 2008 Oct 9.
Information is needed regarding analytical characteristics of cardiac troponin (cTn) assays used in preclinical studies.
We measured cTnI and cTnT in serum from normal animals and animals with induced myocardial injury [Sprague-Dawley (SD) and Wistar rats, beagle dogs, and rhesus (Rh) and cynomolgus (Cy) monkeys]. We evaluated the following assays: for cTnI, Abbott Architect, Bayer Centaur (first and second generation), Beckman Access, DPC Immulite, Dade Dimension, Ortho Vitros ES, Tosoh AIA, and species-specific enzyme immunoassays; for cTnT, Roche Elecsys.
We found different species-specific responses for the troponin assays evaluated. Abbott, Bayer Ultra, Beckman, and Dade assays gave good responses across all species. In rats, weak responses were observed with DPC and Ortho, and no measurable response with Tosoh. In dogs, weak responses were observed with Tosoh cTnI, Roche cTnT, and species-specific cTnI. In cynomolgus monkeys, weak responses were observed with species-specific cTnI and Roche cTnT. Assay imprecision was < or = 20% at 3 or more examined cTn concentrations for Beckman (rat, dog, monkey), Dade (rat, dog, monkey), Abbott (rat, dog, monkey), Bayer first generation (dog), Bayer Ultra (rat, dog, monkey), Roche (monkey), DPC (dog, monkey), Ortho (dog, monkey), and Tosoh (dog, monkey) assays, whereas imprecision was < or = 20% at 2 or fewer concentrations for the Bayer first generation (rat, monkey), Roche cTnT (rat, dog), and DPC (rat) assays.
Not all cTn assays are suitable for monitoring cTn in each animal species or strain. Individual assay characterization by animal species is needed to prevent misinterpretation of myocardial injury-based cardiac troponin findings.
临床前研究中使用的心肌肌钙蛋白（cTn）检测方法的分析特性相关信息十分必要。
我们检测了正常动物以及诱导心肌损伤动物（斯普拉格-道利大鼠、Wistar大鼠、比格犬、恒河猴和食蟹猴）血清中的cTnI和cTnT。我们评估了以下检测方法：对于cTnI，有雅培Architect、拜耳Centaur（第一代和第二代）、贝克曼Access、DPC Immulite、达德Dimension、奥瑟Vitros ES、东芝AIA以及物种特异性酶免疫测定法；对于cTnT，有罗氏Elecsys。
我们发现所评估的肌钙蛋白检测方法存在不同的物种特异性反应。雅培、拜耳Ultra、贝克曼和达德检测方法在所有物种中均有良好反应。在大鼠中，DPC和奥瑟检测方法反应较弱，东芝检测方法无可测量反应。在犬类中，东芝cTnI、罗氏cTnT和物种特异性cTnI反应较弱。在食蟹猴中，物种特异性cTnI和罗氏cTnT反应较弱。贝克曼（大鼠、犬类、猴类）、达德（大鼠、犬类、猴类）、雅培（大鼠、犬类、猴类）、拜耳第一代（犬类）、拜耳Ultra（大鼠、犬类、猴类）、罗氏（猴类）、DPC（犬类、猴类）、奥瑟（犬类、猴类）和东芝（犬类、猴类）检测方法在3个或更多检测的cTn浓度下测定不精密度≤20%，而拜耳第一代（大鼠、猴类）、罗氏cTnT（大鼠、犬类）和DPC（大鼠）检测方法在2个或更少浓度下测定不精密度≤20%。
并非所有cTn检测方法都适用于监测每种动物物种或品系中的cTn。需要按动物物种对各个检测方法进行特性描述，以防止对基于心肌损伤的心肌肌钙蛋白检测结果产生误解。
