Darnton Nicholas C, Berg Howard C
Rowland Institute at Harvard, Cambridge, Massachusetts 02142, USA.
J Bacteriol. 2008 Dec;190(24):8223-4. doi: 10.1128/JB.00908-08. Epub 2008 Oct 10.
There are mutants of Salmonella enterica (with mutations in fliF and fliL) that shed flagella when they are swimming in a viscous medium or on the surface of soft agar. Filaments with hooks and the distal rod segment FlgG are recovered. We tried to extract flagellar filaments from such cells by pulling on them with an optical trap but failed, even when we used forces large enough to straighten the filaments. Thus, flagella are firmly anchored.
存在肠炎沙门氏菌的突变体(fliF和fliL发生突变),当它们在粘性介质中或软琼脂表面游动时会脱落鞭毛。带有钩和远端杆段FlgG的丝状体被回收。我们试图用光学镊子拉动这些细胞来提取鞭毛丝状体,但即使使用足以拉直丝状体的力也失败了。因此,鞭毛锚定牢固。