Department of Physics and Graduate Institute of Biophysics, National Central University, Jhongli, Taiwan, ROC.
Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing, China.
Nat Commun. 2021 Apr 12;12(1):2160. doi: 10.1038/s41467-021-22483-8.
The dynamic assembly of the cell wall is key to the maintenance of cell shape during bacterial growth. Here, we present a method for the analysis of Escherichia coli cell wall growth at high spatial and temporal resolution, which is achieved by tracing the movement of fluorescently labeled cell wall-anchored flagellar motors. Using this method, we clearly identify the active and inert zones of cell wall growth during bacterial elongation. Within the active zone, the insertion of newly synthesized peptidoglycan occurs homogeneously in the axial direction without twisting of the cell body. Based on the measured parameters, we formulate a Bernoulli shift map model to predict the partitioning of cell wall-anchored proteins following cell division.
细胞壁的动态组装是维持细菌生长过程中细胞形状的关键。在这里，我们提出了一种在高时空分辨率下分析大肠杆菌细胞壁生长的方法，该方法通过跟踪荧光标记的细胞壁锚定的鞭毛马达的运动来实现。使用这种方法，我们可以清楚地识别细菌伸长过程中细胞壁生长的活跃区和不活跃区。在活跃区，新合成的肽聚糖沿轴向均匀插入，而细胞体不发生扭曲。基于测量的参数，我们制定了一个伯努利转移图模型来预测细胞分裂后细胞壁锚定蛋白的分配。
